It is intended to mimic the most common cause of obesity in humans. The rats were included in four groups after induction of obesity. Normal group, 10 rats fed on normal diet during the entire study. Highfat dietinduced metabolic syndrome and oxidative stress in. Herzog yz y human performance laboratory, university of calgary, ab, canada z mccaig institute for bone and joint health, university of calgary, ab, canada. Thus, the aim of the present study was to investigate the effect of highfat diet induced obesity on male reproductive parameters. A blood sample was taken from the tail vein before the start of the study at 70 days of age for the measurement of glucose, insulin, and leptin. Using dietinduced obesity to understand a metabolic subtype of osteoarthritis in rats k. Characterisation of ischaemic stroke in dietinduced obese.
Using diet induced obesity to understand a metabolic subtype of osteoarthritis in rats k. Diet induced obese dio mouse models c57bl6ntac male. Measuring conditions include nutritional status of the subject, physical activity and duration of the observation. Proteomic analysis for antiobesity potential of capsaicin. Controlled highfat diet induces an obese syndrome in rats. Apr 01, 2010 changes in expression of skeletal muscle proteins between obesity prone and obesity resistant rats induced by a highfat diet. Individual differences in cueinduced motivation and. The data showed that hfdinduced obesity in lev rats was. After administration, the rats were fed a highfat diet hfd for 9 weeks to induce obesity. In the kidneys of 75weekold rats with continuous hfd consumption, we detected a significant increase in. The effects of diet on body weight are inconsistent such that some studies suggest that a highfat and a highsugar diet will result in weight gain while others suggest that only a highfat diet will lead to. The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. Jul 15, 2015 in the past decades, obesity and associated metabolic complications have reached epidemic proportions.
Pdf dietinduced obesity in zebrafish shares common. Jun 11, 2015 after receiving their respective diet for 10 weeks, the obese induced rats gained significantly higher body weight and bmi than the negative control rats, thus verified to be obese. Lkb1 also improved hfd induced hepatic fatty degeneration. The effect of diet induced obesity on ischaemic stroke cohort 3. The average food and water intake of each rat are presented in table 1. Propensity to highfat dietinduced obesity in rats is. Many previous studies performed on mice and rats suggested that obesity can be induced by high fat diet 21.
Figure 1 demonstrates that diet induced obese rats figure 1a, area under the curve auc. Epigenetic dysregulation of the dopamine system in diet. Highfat diet increases pain behaviors in rats with or. Butyrate may represent a mechanism for the obesityattenuating effects of prebiotics butyrate, a shortchain fatty acid produced by gut microbe fermentation of dietary fiber, was increased in the cecum and colon of these prebiotictreated mice. Longterm hfd leads to damage, inflammation and fibrosis in the kidneys of aging rats. Wistar rats are also used in studies where obesity is diet induced, and the results have shown that the body weight is increased. In four experiments, longevans rats ate ad libitum a. Protective effects of tamarillo cyphomandra betacea extract. The aim of this research was to confirm whether a combination of quercetin and resveratrol cqr could restore the gut microbiota dysbiosis induced by a highfat diet hfd. In general, a highfat hf diet induces the development of metabolic.
Wistar rats are also used in studies where obesity is dietinduced, and the results have shown that the body weight is increased. However, little is known on whether combination of dietary control and ato treatment could enhance the therapeutic effect. Rat models of dietinduced obesity and high fatlow dose. Effects from dietinduced gut microbiota dysbiosis and. Rodent model for the study of obesityrelated disorders 387rev assoc med bras 2012. Typically mice, rats, dogs, or nonhuman primates are used in these models. Modeling dietinduced obesity with obesityprone rats. We aimed to investigate the potential efficacy of hmf on preventing obesity and hyperlipidemia in rats fed a highfat diet hfd and its underlying mechanisms. A total 40 rats were randomly assigned into two groups, normal 10 rats and obese 30 rats.
The aim of this study was to examine the inflammatory status of the kidneys and potential ongoing renal damage, i. Comparison of dietary control and atorvastatin on high fat. In this present study, the high fat diet consists of 40% of fat of total energy. Treating obese and diabetic rats with enalapril or a menhaden oil enriched diet generally improved peripheral neuropathy endpoints.
This study aims to investigate the protective effect of cyphomandra betacea in adult male spraguedawley rats fed with high fat diet. While body weight is typically used in diet induced models of obesity as the measure of obesity in rats, it may not be the best measure. The effect of diet induced obesity on ischaemic stroke cohort 1 figure 14. Vagal nerves are perfectly positioned to convey peripheral metabolic status to the brain. Intraventricular injection of lkb1 inhibits the formation of.
Lkb1 delivery decreased body weight, energy intake, fat mass, and serum lipid levels. Effect of consumption of a highfat hf diet on body weight, adiposity, and food intake in lowfat lf and hffed animals after 8 or 12 wk on respective diets a. Jun 25, 2019 obesity is a risk factor for chronic kidney disease ckd. Highfat dietinduced obesity causes an inflammatory. Treatment with atorvastatin ato or dietary control has been demonstrated to benefit patients with nonalcoholic fatty liver disease nafld and hyperlipidemia. To investigate the potential consequences of hfd induced longterm obesity on kidney damage, inflammation and fibrosis, we first performed comprehensive analyses of passtained renal sections fig. This study was conducted to determine the mechanisms implicated in the beneficial effects of apple polyphenols aps against diet. Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. In addition, after junkfood diet exposure, those rats that developed obesity also showed increased willingness to gain access to a sucrose cue. In this study, it was purposed to examine histological effects of fatty diet induced obesity on liver from female obese rats. Over the past two decades, some confusion has emerged in the terminology used in describing outbred models.
Highfat diet consumption for 8 weeks affected body weight in longevans but not spraguedawley rats. Effect of carnitine and herbal mixture extract on obesity. Obesity is rapidly becoming a worldwide epidemic that affects children and adults. We found that rats susceptible to dietinduced obesity displayed heightened conditioned approach prior to the development of obesity. A controlled highfat diet induces an obese syndrome in rats. In this study, we used the paradigm of diet induced obesity first described by woods et al. Effects of different diets used in dietinduced obesity models on. While body weight is typically used in dietinduced models of obesity as the measure of obesity in rats, it may not be the best measure. As mentioned in table 3, it has been observed that rats feed on highfat diet consecutively for 21 days resulted in a marked increase in the level of lipids, characterized by elevated levels of total cholesterol, triglycerides, ldl, vldl, and reduced levels of hdl when compared to normal control, that is, rats receiving the normal. Dietinduced obesity in rats leads to a decrease in sperm. In comparison with salinetreated rats, body weight of those in the capsaicintreated. High fat diet induced obesity impairs skeletal muscle.
Dietinduced obesity in rats is associated with glucose intolerance during pregnancy but not in the nonpregnant state. Obesity was induced in rats for 70 day by feeding the high fat diet. The sd spraguedawley rat model of dio diet induced obesity is reported to exhibit a clear segregation into susceptible and resistant subpopulations shortly after transfer to a he high energy diet. Control c rats had free access to a standard nonpurified diet containing 51% carbohydrate, 4% fat, and 21% protein. Obesity epidemic warrants scientific attention in the u. Wet weight and cellularity of dissected adipose depots of adult rats fed a highfat diet for 5 mo gonadal bdy wt, retroperitmeal inguinal g total lipid, cell size, pg no. Methods female wistar rats were fed either a standard chow 3% fat, 7% sugar wtwt or a palatable obesogenic diet 11% fat, 43% sugar wtwt for 8 weeks before mating and throughout pregnancy and lactation. Obesity and its comorbidities are currently considered an epidemic, and the involved pathophysiology is well studied. Therefore, we aimed to determine the role that either compound had on body weight and body composition, renal and hepatic function in dietinduced obesity. Using dietinduced obesity to understand a metabolic.
While the exact mechanisms remain unclear, inflammation may be a consequence of obesity that directly impacts the kidneys. Dietinduced obesity in rats leads to a decrease in sperm motility article pdf available in reproductive biology and endocrinology 91. Diet rich in fat induced obesity not only in human but also in animals. Low doses of cocoa extract supplementation ameliorate diet. Thus, the aim of the present study was to investigate the effect of highfat dietinduced obesity on male reproductive parameters. Original article berberine protected rats against adiposity. Characterization of the satellite glial cell sgc in the. Individual differences in cueinduced motivation and striatal systems in rats susceptible to dietinduced obesity mike jf robinson1,2, paul r burghardt3,4, christa m patterson5, cameron w nobile6, huda akil3, stanley j watson3, kent c berridge1 and carrie r ferrario,6 1department of psychology, university of michigan, ann arbor, mi, usa. The role of o1602 and o1918 in attenuating obesity and obesityrelated pathologies is unknown. Here, we demonstrate that supplementation with low doses 14 and 140 mg per kg per rat of a complete cocoa extract induces metabolic benefits in a diet induced obesity dio model of wistar rats. To charles river north america in 1975 from charles river uk.
It is known that obesity is associated with many chronic disorders such as nonalcoholic fatty liver disease nafld. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf diet induced obesity has not been adequately evaluated so far. Mar 11, 2011 obesity is rapidly becoming a worldwide epidemic that affects children and adults. As mentioned in table 3, it has been observed that rats feed on highfat diet consecutively for 21 days resulted in a marked increase in the level of lipids, characterized by elevated levels of total cholesterol, triglycerides, ldl, vldl, and reduced levels of hdl when compared to normal control, that is, rats receiving the normal feed. Longterm characterization of the dietinduced obese and diet. Impact of chromium histidinate on high fat diet induced. Inquire about customized diet induced obese dio models using any of taconics commercial animal models.
In conclusion, our results provide in vivo genetic support for a role for adipose tissue gatm and creatine metabolism in energy expenditure and the defense. Rats were fed on either normal chow or high fat diet for 10 weeks for obesity induction phase and subsequently received c. It is well recognized that capsaicin increases thermogenesis through enhancement of catecholamine secretion from the adrenal medulla. Oct 16, 2009 a total 40 rats were randomly assigned into two groups, normal 10 rats and obese 30 rats. Using dietinduced obesity to understand a metabolic subtype. Diet induced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity. Blood glucose levels of control and high fat diet hfd fed rats cohorts 14. Butyrate has been reported to attenuate dietinduced obesity and insulin resistance. Since rats and mice show a similar relationship, they are considered an appropriate model. Rats fed a chow fat diet were used as normal controls. Daily energy expenditure consists of three components. Obesity is a risk factor for chronic kidney disease ckd.
May 01, 2017 the fact that rats of the cafeteria diet group initially ingested a lower quantity of food feed and cafeteria diet may be related to a compensatory mechanism of the organism in relation to the high energy level of the cafeteria diet. According to another survey, the global prevalence of obesity has. Established dietinduced obesity in female rats leads to. Role of butyrate in restoring satiety signaling in rodent. This obese rat model is an attractive model to study further the physiologic and molecular abnormalities in gdm. In this study, wistar rats were divided into three groups. Body weights of male rats fed the highfat diet hf in expt 1 n 16, expt 2 n 8, expt 3 n 16 and female rats in expt 4 n 8 as a percentage of the body weight of rats fed the lowfat diet lf over 10 wk. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf dietinduced obesity has not been adequately evaluated so far. Custom diet protocols can use any diet manufactured according. In the present study of the antiobesity effect of capsaicin, rats 5week old received capsaicin 10 mgkg along with a highfat diet hfd. Lkb1 also improved hfdinduced hepatic fatty degeneration. Marques c 1, meireles m2, norberto s2, leite j 2, freitas j 3, pestana d1, faria a4, calhau c 1.
Table 1 a different types of diet used and dietinduced body and metabolic alterations dietinduced obesity. Over the last two decades, we and others have employed an outbred rat model to study the development and persistence of obesity, as well as. Aug 24, 2018 recent data demonstrate that hypothalamic activation of the unfolded protein response upr plays a primary pathogenic role in obesity. Therefore, the absence of dietinduced thermogenesis in ko mice in the first days following a highfat diet switch may largely explain their profound susceptibility to dietinduced obesity. When fed ad libitum with a highfat diet hfd, obesity, hyperinsulinemia, hyperglycemia and hypertension establish in these mice. However, little is known about the contribution of 3,5,6,7,8,3. This is likely to result from the additive effects of obesity and pregnancy on insulin sensitivity. Rodents with dietinduced obesity dio are widely used as models of obesity. Hypercaloric diets are tightly related to the obesity etiology and also cause alterations in gut microbiota functionality. The composition and preparation techniques of yogurt are provided in a supplementary file.
Find, read and cite all the research you need on researchgate. A combination of quercetin and resveratrol reduces obesity. Male spraguedawley rats were fed a highfat diet 40% digestible energy from lipids. Diet and antibiotics are known to play crucial roles in changes in the microbiota ecosystem and the disruption of its balance. Ontogeny of dietinduced obesity in selectivelybred rats. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or type 2 diabetes. Diet induced and genetic models of obesity have been. In the past decades, obesity and associated metabolic complications have reached epidemic proportions. Here, we demonstrate that supplementation with low doses 14 and 140 mg per kg per rat of a complete cocoa extract induces metabolic benefits in a dietinduced obesity dio model of wistar rats. This does not appear to be the case for rats sourced in the u. Male sprague dawley rats were fed a westernstyle 21% fat by weight. After 10 weeks, cocoa extractsupplemented animals exhibited significantly lower body weight gain and food efficiency, with no differences in energy.
Current study comprises usage of a dietinduced obesity model by proposing high fat diet on adult female rats and examination of the livers of these obese rats under light microscope. Proteomic analysis for antiobesity potential of capsaicin on. For the study of these pathologies, a number of animal models have been developed. Values are mean sem the proportional increase of body weight of hf rats relative to lf rats followed a comparable trajectory for cohorts studied at different times of the. In a first experiment, male wistar rats were fed a highfat diet hfd. Protective effects of tamarillo cyphomandra betacea. Fatty diet is a significant factor causing obesity. Individual differences in cueinduced motivation and striatal. Antiobesity and hypolipidemic activity of moringa oleifera. This particular colony was selected because of a low incidence of hydronephrosis. Measuring conditions include nutritional status of the subject, physical activity and duration of. Intraventricular injection of lkb1 inhibits the formation. Pdf dietinduced obesity in rats leads to a decrease in sperm.
One such polygenetic model is the dietinduced obese dio and dietresistant dr outbred rat models levin et al. Rats on a cafeteriastyle caf diet 10 had free access to a semisolid diet containing 33% ground commercial rat chow, 33% full fat sweetened condensed milk nestle, 7% sucrose, and 27% water. Development of a new dietinduced obesity dio model using. Request pdf on sep 1, 2001, mr ricci and others published ontogeny of dietinduced obesity in selectivelybred rats. Some studies have shown a relationship between obesity and infertility, but until now it remains controversial. Therefore, the absence of diet induced thermogenesis in ko mice in the first days following a highfat diet switch may largely explain their profound susceptibility to diet induced obesity. The effect of diet induced obesity on ischaemic stroke cohort 2 figure 15. In this study, we used the paradigm of dietinduced obesity first described by woods et al.
Effect of carnitine and herbal mixture extract on obesity induced by high fat diet in rats. Diet induced obese dio mouse models c57bl6ntac male mice. We employed a rat model of nafld to examine the therapeutic efficacy of. The fact that rats of the cafeteria diet group initially ingested a lower quantity of food feed and cafeteria diet may be related to a compensatory mechanism of the organism in relation to the high energy level of the cafeteria diet. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. Pdf dietinduced obesity in rats leads to a decrease in. Control males are housed in the same location, also at reduced density, and are fed nih31m diet control males can also be generated using a low fat purified diet upon request. However, its metabolic role in the hypothalamus remains unknown.
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